Showing 5 results for Asadi
Mahdieh Asadi, Parvin Farzanegi,
Volume 2, Issue 1 (5-2014)
Abstract
Background & Objective: High blood levels of IGF-1 in women before menopause, is a risk factor and P53 tumor suppressor gene is also one of the most common genes involved in breast cancer. Since some studies demonstrated that physical activity and compounds found in Celery have anti-cancer effects, this study aimed to investigate the interactive effects of eight weeks massage therapy along with celery seed supplementation on the levels of IGF-1 and P53 in overweight women.
Method: In a single, blind, placebo-controlled clinical trial, the interactive effects of eight weeks massage therapy along with celery seed consumption on IGF-1 and P53 serum concentrations in overweight women were measured. For this purpose, 28 overweight women with a BMI of more than 25 kg/m2 in Ghaemshahr were randomly divided into four groups. Weight and serum levels variables of IGF-1 and P53, prior and after the therapy were measured. Then the data were analyzed using t-test and one-way variance analysis. P<0.05 was considered as the level of significance
Results: At the end of the study, the average of weight and level of IGF-1 and P53 in experimental groups (massage therapy, Therapy and supplementation, Supplementation) were decreased. There was a significant difference between the weight (P=0.003), the level of IGF-1 (P <0.001) and P53 (P<0.001) of women before and after the start of the intervention in the experimental group while the control group was not changed.
Conclusion: The results show that massage therapy along with celery seed supplements, especially the combination of these two non-pharmaceutical approaches have beneficial effects on body weight and
IGF-1 and P53 levels in overweight women.
Ayyoob Khosravi, Fariba Kokabi, Ramezan Behzadi, Jahanbakhsh Asadi,
Volume 7, Issue 3 (9-2019)
Abstract
Background and objectives: Modeling cancer in vivo is a very important tool to investigate cancer pathogenesis and molecular mechanisms involved in cancer progression. Laboratory mice are the most common animal used for rebuilding human cancer in vivo. Cancer stem cells (CSCs) are the main reason of failure in cancer therapy because of tumor relapse and metastasis. Isolation of cancer stem cells helps us to study their function and behavior. In the current study we separate cancer stem-like cells using sphere formation assay then investigate their tumorigenicity in xenograft tumor model.
Methods: YM1 cancer cells were cultured in serum-free media (SFM) in low adherent culture dishes for enrichment of cancer stem cells. The resulting spheres containing cancer stem-like cells were dissociated into single cells and were injected into the dorsal flank of B6 nude mice.
Results: A few days after injection, subcutaneous tumors formed. The growth curves of the resulting tumors were plotted using their weekly recorded lengths. The tumors' volume and weight were measured. The size of resulting tumors was appropriate to the number of cells injected. Pathological analysis confirmed esophageal origin of the resulting tumors.
Conclusion: Using laboratory mice models is a practical modeling system that provides us investigation of human tumors pathogenesis in vivo.
Arezoo Monfared, Mohammad Taghi Moghadamnia, Samad Karkhah, Saman Maroufizadeh, Mohammad Asadian Rad, Jalal Kheirkhah, Fatemeh Jafaraghayee,
Volume 9, Issue 4 (12-2021)
Abstract
Background and Objective: In the current COVID-19 pandemic, disease diagnosis is essential for optimal management and timely isolation of infected cases in order to prevent further spread. The aim of this study is to assess of predictors of mortality among COVID-19 patients.
Material and Methods: In a retrospective study, 522 COVID-19 patients were enrolled in Razi hospital, Guilan Province, Iran. This hospital was the main center for the treatment of COVID-19 patients in Guilan province. Data gathering was performed by census sampling from March to August 2020. Simple and Multiple logistic regression analysis was applied to assess the relationships of clinical and demographic characteristics with in-hospital mortality.
Results: Multiple logistic regression showed that older age (aOR=1.04, 95%CI: 1.02 to 1.06, P<0.001), decreased O2 saturation (aOR=0.89, 95%CI: 0.86 to 0.92, P<0.001), having a dysrhythmia (aOR=2.97, 95%CI: 1.46 to 6.05, P=0.003), symptoms associated with heart failure (aOR=0.43, 95%CI: 0.18 to 0.99, P=0.048), and mixed drug antiviruses (aOR=2.44, 95%CI: 1.22 to 4.90, P=0.012) were mortality predictor variables among COVID-19 patients.
Conclusion: Therefore, special attention should be paid to the factors influencing the mortality of COVID-19 patients. It is recommended that older patients, dysrhythmia, and symptoms associated with heart failure be treated with extreme caution.
Zeinab Mohammadi, Marie Saghaeian Jazi , Seyyed Mehdi Jafari , Seyed Mostafa Mir , Massoud Amanlou , Jahanbakhsh Asadi ,
Volume 12, Issue 4 (12-2024)
Abstract
Background: Bone remodeling involves a balance between osteoblast-driven formation and osteoclast-mediated resorption, with disruptions leading to diseases like osteoporosis. Midazolam (MDZ), known for its sedative properties, has shown effects on cellular differentiation and hydroxyapatite formation in dental cells. However, its role in promoting osteogenic differentiation in human bone marrow mesenchymal stem cells (hBMSCs) remains unexplored, motivating this study to investigate its potential in bone regenerative therapies.
Methods: Human bone marrow stem cells were cultured in the complete media with α-MEM, 10% FBS, and 1% pen/strep. Cell viability was determined with MTT assay in different concentrations of MDZ (0.125 to 1 µM) for 72 hours. Osteogenic differentiation was induced over 21 days using the selected doses of MDZ with osteogenic medium. The Alizarin Red S (ARS) staining was performed to determine the calcium deposit for osteoblast cells. Data were analyzed using repeated measure ANOVA, and a p-value <0.05 was considered statistically significant.
Results: The MTT results for different concentrations of MDZ (0.125 to 1 µM) showed no significant cytotoxic effects on hBMSCs after 72 hours. Furthermore, ARS staining revealed increased calcium deposits in 0.5 µM MDZ compared to untreated and DMSO groups (P-Value =0.0001). These findings suggest that MDZ promotes mineralization at lower concentrations, highlighting its potential in osteogenic applications.
Conclusion: Midazolam promotes osteogenic differentiation of hBMSCs, particularly at 0.5 µM concentration, without cytotoxic effects. These findings demonstrate that MDZ may be a potential compound for osteoblastogenesis; however, these findings require further in vivo studies to confirm the idea.
Jahanbakhsh Asadi , Asra Askari , Zeinab Mohammadi , Babaisan Askari ,
Volume 13, Issue 2 (6-2025)
Abstract
Background: The higher occurrence of NAFLD is associated with insulin resistance, obesity, and diabetes. A diet with high fat or sucrose serves as a NAFLD inducer in rats, which exhibits significant variability and generally results in low levels of liver inflammation and fibrosis. Our study highlights the role of combining animal fats with high sucrose in inducing NAFLD in Wistar rats.
Methods: Twenty male Wistar rats, 8 to 10 weeks old, were randomly divided into two groups: Control (healthy) and NAFLD induction. Weekly weight changes were recorded. After one week of adapted feeding, a 30% vegetable oil and 10% solid sugar diet was added to standard pellets to induce the NAFLD model. After ten weeks, the weight/time of progression of each mouse was measured. Serum and tissue samples were separated and stored for biochemical and histopathological studies. Comparisons were made using independent t-test for two groups with SPSS version 22. The significance level was set at 0.05.
Results: Our results illustrated that weight/time progress increased in the NAFLD-induced group (0.679 g ± 0.02, P < 0.001) more than in the control group (0.559 g ± 0.03). Additionally, FBS (P = 0.001), lipid profile [TG/HDL (P = 0.006), LDL/HDL (P = 0.03), Chol/HDL (P = 0.006), TG (P = 0.005), CHOL (P = 0.001), LDL (P = 0.008), VLDL (P = 0.005), HDL (P = 0.01)], liver function enzymes [ALT (P = 0.001), AST (P = 0.001)[ and IL-6 serum levels were significantly increased in the NAFLD-induced group compared to control rats (P = 0.001). Besides, the obtained results illustrated that the serum level of albumin was significantly decreased in NAFLD-induced rats compared to controls (P = 0.004). Moreover, Oil Red and H&E staining confirmed grade two steatosis induction.
Conclusion: Our study demonstrates significant metabolic abnormalities and steatosis in NAFLD-induced rats, underscoring the detrimental impact of this disease on liver function and overall health. These findings highlight the urgent need for further research into effective interventions for NAFLD.
