M Rasouli Nasab, Sh Habibnia, P Heidarieh, Mr Pourmand, M Fatahi, Ss Eshraghi,
Volume 7, Issue 5 (supplement Issue( Bacteriology)[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: The Isolation of Nocardia species is complex and time-consuming, which is due to rapid growth of adjacent bacteria. Because of the importance of a specific medium with the ability of controlling intrusive microorganisms, this study aimed at comparing three laboratory methods to introduce the reliable isolation technique for Nocardia species.
Material and Methods: The soil samples were collected from different regions of Tehran province, Iran, and carefully transferred to the laboratory. The samples were cultured in three different media including Paraffin Baiting,Humic acid vitamin B agar and Paraffin agar, and incubated for 3-4 weeks at 35 °C.
Results: Of 110 soil samples, 31 Nocardia isolates (28.18%) were obtained from the media including Paraffin Baiting, (19 17.27%), Humic acid and vitamin B agar (4 3.63%), and Paraffin agar, (8 7.27%).
Conclusion: because of high rate of isolation, low cost and the clearance of colonies suspected nocardia, Paraffin Bait technique is more reliable and efficient compared to the other methods.
Key words: Nocardia Soil Paraffin Baiting Humic Acid Vitamin B
Masoud Sheikh , Hamid Reza Eshraghi, Masoud Khoshnia, Masoumeh Mazandarani, Abdolvahab Moradi,
Volume 11, Issue 4 (Jul-Aug 2017)
Abstract
ABSTRACT
Background and Objectives: Capparis spinosa has been used in traditional medicine for various applications including treatment of liver disorders and cancer. We studied the effects of this plant on cell proliferation and morphological characteristics of
PLC/PRF/5 liver cancer cell line.
Methods: After preparing ethanolic extract of the plant, the inhibitory effect of the extract was assessed using MTT assay, and morphological changes were assessed by an inverted microscope.
Results: C. spinosa ethanolic extract exhibited anti-cancer effects in a concentration-dependent manner. Half-maximal inhibitory concentration of the extract was 1051±4.21 mg/mL. Morphological changes including cell shrinkage, reduction of cell volume and nuclear condensation confirmed the inhibitory effect of
C. spinosa on
PLC/PRF/5 cells.
Conclusion: According to the results of this study, extract of C. spinosa seems to be suitable for prevention and treatment of liver cancer. Further studies on animal models could verify the efficiency of the extract against cancer cells.
KEYWORDS: Plants, Medicinal, Capparis spinosa, Liver Neoplasms.
