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Showing 2 results for Mahboubi
Antibiotic Resistance of the Isolated Bacteria from Blood Cultures of Cancer Patients
Asghari Estiar, M, Rafi, A, Heidarzadeh, S, Ohadian Moghadam, S, Mahboubi, R, Monadi Sefidan, A, Allafzadeh, J, Nik Khah, H,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract

Abstract Background and Objective: One of the main causes of increased mortality in cancer patients is bacteremia. On the other hand, antibiotic resistance is the major cause of treatment failure in malignant diseases especially in hematological malignancies. The aim of this study was to diagnose the bacterial strains isolated from blood specimens of cancer patients and to determine their antibiotic susceptibility. Material and Methods: In this cross-sectional study, 0.5 ml of venous blood was taken from 613 cancer patients especially leukemia, and blood cultures and antibiotic susceptibility tests were performed using standard methods. Using disc diffusion method, antibiotic susceptibility was performed with a wide range of antibiotics. Results: Out of 613 cultured specimens, 153 (25%) were found to be positive including 76.47% of gram negative and 23.53% of gram positive bacteria. The most common isolated bacteria were E. coli, coagulase-negative Staphylococci, Klebsiella, Staphylococcus aureus and Pseudomonas aeroginosa, respectively. Conclusion: It seems that Ceftriaxone is the best choice for the treatment of gram negative caused bacteremia and Gentamicin for bacteremia caused by gram positive agents. Given the high level of resistance to the commonly used antibiotics, it seems reasonable to avoid of early and inappropriate use of antibiotics to prevent the development of drug resistant bacteria. Keywords: Cancer, Blood Cultures, Bacteremia, Antibiotic Resistance


Cloning, expression, and purification of recombinant Enterotoxin B and cholera toxin B fusion protein in Lactobacillus plantarum
Fatemeh Maghsood Ahmadi , Arash Mahboubi , Farzaneh Hosseini , Davoud Esmaeili , Bahareh Hajikhani ,
Volume 19, Issue 5 (Sep-Oct 2025)
Abstract
Background: Lactic acid bacteria (LAB) are promising platforms for mucosal vaccine development. Staphylococcus aureus enterotoxin B (SEB), a potent superantigen, is associated with food poisoning and toxic shock syndrome. Similarly, cholera toxin is the primary to widespread virulence factor in Vibrio cholerae infections, with its B subunit (CTB) serving as a well-established immune adjuvant that enhances antigen-specific responses in recombinant vaccines. This study aimed to engineer recombinant Lactobacillus plantarum as a dual-purpose vaccine candidate targeting V. cholerae and S. aureus by expressing CTB and SEB antigens.
Methods: A modified gene sequence encoding SEB (Lacking superantigenic activity) and CTB was successfully designed, synthesized, and cloned for secretory expression in L. plantarum. The resulting recombinant protein, tagged with His, was purified using Ni-NTA agarose ion-exchange chromatography and confirmed with Western blot analysis.
Results: Enzyme digestion and PCR analysis confirmed successful cloning of the SEB-CTB fusion gene into the pBlueScript II SK (+) and pNZ7021 expression vectors, as evidenced by the expected band on agarose gel. SDS-PAGE revealed a ~49 kDa protein band, indicating expression of the recombinant rSEB-CTB protein, which was further validated by Western blot using an anti-His tag antibody.
Conclusion: The construct LP-pNZ7021–SP-seb-ctxB may be a promising candidate for recombinant vaccine development targeting V. cholerae (Cholera toxin-producing) and S. aureus (SEB-producing), providing dual protection against both pathogens.
 

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