Showing 6 results for Shafi
Shahande Z, Sadighian F, Shafi H, Ebrahimnejad A,
Volume 5, Issue 1 (spring-summer[PERSIAN] 2011)
Abstract
Abstract Background and objectives: Infection stones are the directly due to persistent or recurrent infection with Urease producing bacteria. they may be exacerbated by urinary obstruction or stasis. Also, some drug components like ciprofloxacin can be the cause of stone formation. We aimed at studing antibiotic resistance of bacteria isolated form kidney stones and Urinary tract infection(UtI). Material and Methods: This descriptive study, during 2008-2010,we obtained kidney stones and urine of 45 patients under gone nephrolithotomy in shahid Beheshti hospital of Babol, Iran. The stones were transferred to microbiology Lab in a strile Condition, after grinding, cultured on Blood Agar and Eosin-Methylene Blue(EMB) media. In Addilion, urin Samples were cultured on the for mentioned media. The Positive cultures were qualitatively evaluated and then, antibiogram was done by using Kirby Bauer method. Results: The resulte show that 10 kidney stone specimens and 8 urine samples were infected by bacteria, mostly by E.coli. In one stone sample and two urine samples, we can isolate more than one bacteria. The bacteria isolated in the stone and urine samples of six patients was the same. We could find two cases of staphylococcus inurine and one in stone culture. The bacteria isolated from stone are resistance to oflaxacin(80%) , which is higher than that to the other antibiotics. Conclusion: Based on the results, there is no relationship between the kind of kidney stone and type of bacteria. Further study needs to be done to prove this relation. Key words: Kidney stone, Antibiogram, E.coli, Infected stone.
H Tavilani, R Setarehbadi, A Fattahi, Sh Nasrollahi, J Karimi, G Shafiee, M Hosseinipanah,
Volume 7, Issue 4 (winter[PERSIAN] 2014)
Abstract
Abstract
Background and objective: There is increasing evidence for the role of oxidative stress in female reproductive tract. The purpose of this study was to determine the activity of antioxidant enzymes during menstrual cycle. In addition, the relationship between activity of antioxidant enzyme and sex hormones was evaluated.
Materials and methods: In this study the activity of superoxide dismutase, glutathione peroxidase, glutathione reductase, catalase and total antioxidant capacity during the menses, follicular and luteal phases of the menstrual cycle in twenty women with regular menstrual cycle were studied. Furthermore, the correlation between activity of antioxidant enzymes and estradiol, progesterone, LH, FSH and testosterone were evaluated.
Results: There was no significant difference between activity of superoxide dismutase, glutathione peroxidase, glutathione reductase, catalase and total antioxidant capacity during the menses, follicular and luteal phases of the menstrual cycle (P>0.05). We found significant correlation, in luteal phase, between superoxide dismutase and FSH (P<0.05، r=0.44) and LH P<0.05،r=0.54). Also it is observed between LH and glutathione peroxidase (P<0.05، r=0.44).
Conclusion: Based on the results, there is no significant difference between antioxidant enzymes and total antioxidant capacity of plasma during menstrual cycle. In other words, physiologic system of women with regular menstrual cycle can protect body against oxidative stress and this is probably performed due to action of FSH and LH hormones.
Keywords: Antioxidants Menstrual cycle Sex hormones
Shafiee, F, Khosravi, Ad, Azarpira, S, Babaie Barkalaie, A, Abbasi Montazeri, E,
Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)
Abstract
Background and Objectives: Pseudomonas aeruginosa is the most common organism, which is separated from the burn infections. Due to increased antibiotic resistance, there are many problems to deal with the infections caused by Pseudomonas aeruginosa. This study aimed to determine the resistance to antibiotics against clinical isolates of Pseudomonas using phenotype methods.
Material and Methods: 100 strains of Pseudomonas aeruginosa were collected from the burn patients in Taleghani hospital in Ahwaz, Iran, during a six-month period. After phenotypically initial identification, antibiotic sensitivity of isolated strains to conventional antibiotics against Pseudomonas aeruginosa was determined using a disk diffusion technique, and Phenotypic screening for MBLs production was performed.
Results: the maximum percentage was related to wound infection and the frequencies of the resistance to imipenem, meropenem, piperacillin, piperacillin-tazobactam, ceftazidime, gentamicin, amikacin, and ciprofloxacin, doripenem, ertapenem and colistin sulphate, were 70%, 53%, 83%, 67%, 91%, 88%, 84%, 84%, 33%, 90%, and 0%, respectively. The use of CD Test methods was approved for determining resistance to Carbapenems.
Conclusion: antibiotic resistance to Pseudomonas aeruginosa is increasing and colistin sulphate is the most effective antibiotic.
Keywords: Pseudomonas Aeruginosa; Burn Infection; Antibiotic Resistance.
Reyhaneh Shafieian, Alireza Ebrahimzadeh-Bideskan, Esmail Ranjbar,
Volume 18, Issue 3 (May-Jun 2024)
Abstract
Background: The purpose of tissue processing is to strengthen the tissue and place it in a suitable environment for slicing without causing damage. In routine histopathology, decalcification of calcified tissues is a principal step before tissue processing. Thus, the aim of this study is to evaluate the effect of several decalcifying agents on morphological and antigenic preservation in canine mandibular tissue.
Methods: Four different decalcifying solutions, including 5% nitric acid (NA), 10% and 20% formic acid (FA), and 10% ethylene diamine tetraacetic acid (EDTA), were employed to remove mineral salts from specimens harvested from mandibular osseous tissues of four healthy Mongrel dogs (32 samples). The solutions were compared regarding the decalcification time, ease of tissue slicing, morphological organization after Alizarin red S staining, and osteocalcin antigenic preservation.
Results: Considering the short decalcification time and ease of sectioning, 5% NA) yielded better results than 10% and 20% FA and 10% EDTA (pH 7.4). In terms of the preservation of morphology and antigenicity of the tissue samples, 10% EDTA was found to be the most optimal solution, followed by 10% and 20% FA and 5% NA.
Conclusion: Our findings support EDTA as a highly preferred choice for the decalcification of canine osseous tissue when aiming for immunohistochemistry, despite its time-consuming nature. However, for general histological staining procedures, 20% FA and 5% NA are preferred.
Syed Majid Ali, Jalila Qayoom, Talat Masoodi, Azhar Shafi, Arshi Syed,
Volume 18, Issue 5 (Sep-Oct 2024)
Abstract
Background: Post-operative wound infection has been a problem since surgery was started as a treatment modality and is the third most common cause of nosocomial infections with a reported incidence rate of 14-16%. This study aimed to investigate the prevalence, isolate and identify aerobic pathogenic bacteria from surgical site infections (SSI) and to determine the antibiotic susceptibility testing (AST) pattern of pathogenic bacteria.
Methods: This study was conducted at the Department of Microbiology SKIMS-Medical College, Bemina Srinagar, over a period of six months from November 2021 to April 2022. In the study, 210 samples from patients with SSI were included. Isolation, identification, and AST of the isolates were performed by standard microbiological techniques.
Results: Out of 210 SSI samples, 163 bacterial isolates were recovered and infection rate was more in 21-30 years age group (24.2%). Gram-negative bacteria were isolated in 50.4% (82/163) cases and E. coli was the most common organism (59.75%, 49/82). Gram-negative bacteria were sensitive to imipenem and none were resistant to polymyxin-B and colistin. E. coli was mostly resistant to cefoperazone, Acinetobacter and Klebsiella species were resistant to ceftazidime, Pseudomonas and Citrobacter were resistant to ceftriaxone. Gram-positive bacteria constituted 49.6% (81/163) and Methicillin-resistant Staphylococcus aureus (MRSA) was a frequently isolated species (66.6%, 54/81). MRSA- and methicillin-sensitive Staphylococcus aureus (MSSA) were mostly sensitive to amikacin, gentamycin, and tetracycline. Moreover, none of the Gram-positive isolates were resistant to linezolid, vancomycin, and teicoplanin. Enterococcus spp was mostly resistant to gentamycin.
Conclusion: This study developed an insight into post-operative wound infections and their incidence, organisms’ prevalence, and their antibiogram. Culture positivity in suspected cases of SSI was high (77.6%). MRSA and E. coli were frequently isolated from Gram-positive and Gram-negative bacteria.
Zahra Askari, Zeynab Mirzapour, Tooba Shafighi, Reyhaneh Ghorbanpour,
Volume 19, Issue 1 (Jan-Feb 2025)
Abstract
Background: Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) represent a significant global health concern. Virulence factors (VFs) expressed by UPEC strains play a crucial role in promoting bacterial pathogenicity within the urinary tract. Effective treatment of these infections is frequently complicated by the high prevalence of antimicrobial resistance exhibited by Escherichia coli. The objective of this study was to investigate the VFs and antibiotic susceptibility profiles of UPEC strains isolated in the northern region of Iran.
Methods: One hundred and five urine specimens were collected from female patients diagnosed with UTIs in Rasht, located in the north of Iran. These samples underwent culturing on both Eosin Methylene Blue (EMB) agar and MacConkey agar. Following a 24-hour incubation period at 37°C, pure bacterial isolates were identified through Gram staining and a battery of standard biochemical assays. The prevalence of six VF genes - papC, sfa/foc, fimH, afa, ibeA, and neuC - within UPEC strains was determined utilizing polymerase chain reaction (PCR) and subsequently confirmed via direct sequencing. Antibiotic susceptibility testing (AST) was conducted using the disk diffusion method, adhering to the guidelines established by the Clinical and Laboratory Standards Institute (CLSI M02).
Results: The study identified 65.71% of the isolates as Escherichia coli. Among the virulence genes examined, fimH exhibited the highest prevalence (100%), while afa was the least frequent (1.44%). Antibiotic resistance analysis revealed the highest rate against Cefazolin (66.66%) and the lowest against Gentamicin (24.63%). Notably, the prevalence of multi-drug resistance (MDR) was determined to be 73.91%.
Conclusion: This study underscored the significance of localized surveillance of UPEC isolates. This emphasis stems from the pathogen's considerable capacity for genetic mutation, coupled with the influence of environmental variables and individual patient characteristics. Understanding these dynamic factors at a local level is crucial for formulating the most effective strategies to combat UTIs.
