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Showing 4 results for Shaki
Prevalence of E. coli O157: H7 Isolated from Patients with Acute Gastroenteritis
Safaari, M, Zolfaghari, Mr, Shakib, P, Rouhi, S,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Diarrheal diseases may occur in all age groups, worldwide. Escherichia coli (E. coli O157: H7) is one of the most important bacterial agents causing this disease. The purpose of this study was to assess the prevalence of diarrhea in patients with acute diarrhea caused by this bacterium. Material and Methods: In this study, 214 stool samples were collected from acute diarrheic patients in hospitals and clinical laboratories of Malayer city, Iran. The samples were cultured in rich, differentiation, specific and selective medium. To detect E. coli O157: H7, rapid detection method and antisera were used . Results: In 12.15% of the samples, lactose fermentation was observed and E. coli was afirmed. The results of lactose positive samples showed that 6.54% were negative-β- Glucuronidases and were confirmed as E. coli O157. Finally, by using O157: H7 antisera, 1.87% of 14 samples that were negative- β-Glucuronidases were asserted as E. coli O157. Conclusion: based on the results, the prevalence of E. coli O157: H7 is higher in children and elders than the others. Food and water play a significant role in transferring E. coli. Keywords: Prevalence, E. coli O157: H7, Gastroenteritis, Patients
Biosynthesis of Silver Nanoparticles by Fusarium solani isolates from Agricultural Soils in Gorgan, Iran
Hamidreza Pordeli, Hadi Shaki, Ania Ahani Azari, Muhammad Sadeqi Nezhad ,
Volume 12, Issue 4 (Jul-Aug 2018)
Abstract
 

ABSTRACT

            Background and objectives: Silver nanoparticles (AgNPs) are major nanomaterials with a variety of applications. The synthesis of nanoparticles by conventional methods is challenging and often requires use of hazardous chemicals. Therefore, there is a growing need for development of environmentally and economically friendly processes for the synthesis of nanoparticles. This study aimed at biosynthesis of AgNPs using a filamentous fungus; Fusarium solani.
            Methods: Twenty-four Fusarium isolates were found from several soil samples collected from depth of 1-10 cm. All isolates were identified as F. solani based on morphological characteristics. The synthesis of nanoparticles were evaluated after 24, 48, 72 hours of culture. AgNPs were characterized using UV-visible spectroscopy and transmission electron microscopy.
            Results: The synthesized AgNps showed maximum absorbance peak at 420 nm after 72 hours. Moreover, most AgNps were spherical with diameter of between 20 and 40 nm.
            Conclusion: In this study, we introduced a simple biological process for biosynthesis of AgNPs using F. solani isolates from soil samples. The results indicate that fungi may be suitable for safe and cost-effective production of AgNPs.
            Keywords: Fungi; Fusarium; Nanoparticles; Nanotechnology.

Fluconazole and Ibuprofen Combination: A Potential Treatment for Mucosal Candidiasis
Tahere Shamoushaki, Leila Fozouni,
Volume 14, Issue 5 (Sep-Oct 2020)
Abstract
Background and objectives: The incidence of drug-resistant candidiasis has increased dramatically. This study aimed to evaluate antifungal effects of fluconazole alone and in combination with ibuprofen on isolates from patients with mucosal candidiasis.
      Methods: Candida species isolates from 142 patients with suspected mucosal (oral and vaginal) candidiasis were identified by culture on CHROMagar Candida medium and carbohydrate assimilation test using the API 20CAUX kit. Minimum inhibitory concentration (MIC) of fluconazole alone and in combination with ibuprofen was determined by the broth microdilution method.
      Results: Among isolates, 43.7% were identified as Candida species (Candida albicans, Candida glabrata, Candidia parapsilosis, Candida tropicalis, Candida guilliermondii and Candida kefyr). The highest rate of fluconazole resistance was observed among C. albicans (50%) isolates. MIC90 of ibuprofen-fluconazole combination against C. albicans isolates was 32 μg/mL, which was 8-fold less than that of fluconazole alone (MIC90=256 μg/mL) (P<0.01). Moreover, the MIC90 of fluconazole-ibuprofen combination against C. parapsilosis isolates was 4-fold less than that of fluconazole alone.
      Conclusion: Our results revealed partial fluconazole resistance among Candida isolates from patients with mucosal candidiasis. However, the resistance rate decreased 2.5 fold following treatment with the ibuprofen-fluconazole combination. Therefore, it is recommended to further investigate the therapeutic potential of this drug combination for treatment of fungal infections, such as candidiasis.
Investigation of the Frequency and Antimicrobial Susceptibility Patterns of Bacterial Isolated from Blood Cultures in Khorramabad – 2018
Shahnaz Halimi, Pegah Shakib, Elham Mehrabnejad, Faranak Rezaei, Somayeh Delfani,
Volume 19, Issue 6 (11-2025)
Abstract
Background and objectives: Today, the widespread increase in the prevalence of antibiotic resistance in bacteria is considered one of the biggest problems in the health and treatment system around the world. Therefore, this study was conducted with the aim of investigating the prevalence and antibiotic resistance pattern of bacteria isolated from blood cultures of Shahid Rahimi Hospital, Khorramabad in 2018.
Methods: In this cross-sectional study, blood cultures of patients admitted to Shahid Rahimi Hospital from June to December 2018 were investigated. The disc diffusion method was performed according to CLSI instructions. Finally, the data were analyzed using SPSS software.
Results: In the present study, out of a total of 2591 blood culture samples, 247 blood culture samples were positive. The highest frequency was related to the emergency department (62%) and ICU (23%), and the lowest frequency was related to the children's department (2.4%). Among the tested samples, 28% Gram-negative bacilli and 72% Gram-positive cocci were reported. The most abundant Gram-negative and Gram-positive bacteria were Escherichia coli (38%) and Staphylococcus epidermidis (54.2%), respectively. Among Gram-negative bacteria, the highest level of resistance was observed in Acinetobacter baumannii strains, which showed resistance to all antibiotics. Among Gram-positive bacteria, the highest resistance of Streptococcus group D strains to the antibiotic erythromycin (92%) was reported.
Conclusion: The prevalence of antibiotic resistance in common microorganisms isolated from blood cultures in different departments is worrying, and choosing an effective drug to eliminate these bacteria by performing accurate laboratory tests is of particular importance.
 
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