Showing 3 results for Tabarraei A
Livani S, Mirinargesi M, Nemati-Shoja E, Rafiei S, Taziki M, Tabarraei A, ,
Volume 5, Issue 2 (Autumn – Winter 2011[PERSIAN] 2011)
Abstract
Abstract Background and objectives: Identification and monitoring of multidrug-resistant Mycobacterium tuberculosis strains (MDR) is highlighted by the high risk of their spreading in different areas. Prevalence of these strains was evaluated in Golestan province in northeast of Iran. Material and Methods: Drug susceptibility testing to Isoniazid and rifampin was carried out for 148 clinical samples that had grown in Mycobacteria growth indicator tube (MGIT) system, according to the manufacturer's instructions (Becton-Dickinson, USA). The association of drug resistance frequency with demographic characteristics and growth time were investigated. The appropriate statistical tests, X2 and student T- test were performed for comparison of these variants. A p value>0.05 was considered significant in all cases. Results: The turnaround time required for growth of Mycobacterium tuberculosis in MGIT system was between 2 to 55 days (mean 16.3±10.4 days). Of all samples studied, 17.6% and 3.4% were resistant to Isoniazid and rifampin, respectively, and 3.4% (5 samples) were MDR (CI 95% 1-6%). The turnaround time required for determining MDR cases was 9.6 days. No statistically significant association was found between the resistance to the drugs and none of the factors including sex, age, type of clinical sample, and positivity of the smear. Conclusion: The prevalence of MDR in the studied region was determined to be 3.4% which is similar to the country-wide evaluations. The turnaround time for Mycobacterium growth and anti drug susceptibility result can be shortened by MGIT method. Key words: Mycobacterium tuberculosis, Mycobacterium Growth Indicator Tube, Multidrug Resistant
Moradi Av, Azadfar S, Fatemehcheraghali, Javid N, Ghaemi A, Tabarraei A,
Volume 5, Issue 2 (Autumn – Winter 2011[PERSIAN] 2011)
Abstract
Abstract Background and objectives: Mumps virus is one of the first known causative agents of meningitis in children. On-time diagnosis is the first step in treating meningitis. We aimed to evaluate Mumps virus meningitis in children in Gorgan, Iran Material and Methods: CSF and blood samples were taken from children with meningitis, Jun 2008 till Sep 2010. For 40 samples with negative bacterial culture, Extraction of viral RNA was carried out and Real-time PCR was performed for detection of Mumps virus. Demographic, clinical, biochemical and cytological data were collected. We run SPSS version 18 to analyze the data, using Chi Square (p<0.05). Results: three (7.5 %) samples have Mumps virus, two boys and one girl. All three positive cases have 0.5-1 degrees Celsius fever and vomiting but no bulging fontanel. They have not Kernig, Rodor, Brudzinski’s sign, hepatosplenomegaly, lymphadenopathy, pharyngitis and rash. ESR is higher than normal in all positive cases and CRP is positive in two cases. Protein of CSF in one case is higher than normal range. Conclusion: meningitis is an emergency condition therefore, molecular diagnostic techniques are recommended for early diagnosis and intervention. Key words: meningitis, mumps virus, cerebrospinal fluid, Real-Time PCR
Abbasi A (md), Tajbakhsh R (md), Kabotari M, Zhand S (msc), Tabarraei A (phd), ,
Volume 6, Issue 1 (spring-summer[PERSIAN] 2012)
Abstract
Abstract Background and objectives: Hepatitis B virus infection is a major health problem in worldwide. The prevalence of Occult and chronic HBV in hemodialysis patients is higher than standard in developing countries. People with occult HBV are negative for HBV surface antigen (HBsAg) but positive for HBV-DNA. We aimed to evaluate occult hepatitis B infection in patients under hemodialysis in Panje-Azar hospital in Gorgan. Material and Methods: In this study, taken place from 2009 to 2010, the participants were 100 hemodialysis patients with administration of complete HBV vaccination with negative test for HBsAg. After preparing 10 milliliter blood sample, HBV DNA testing was performed by polymerase chain reaction (PCR). Result: The mean age of the patients is 54.60 years. They are male (48%) and female (52%). They have been under hemodialysis for 48 months, averagely. There has not been any HBV-DNA in HBsAg negative patients under hemodialysis. The rate of occult hepatitis B infection in these end stage renal disease (ESRD) patients was zero. Conclusion: Results indicate that there is no any occult HBV infection in ESRD patients under hemodialysis in Gorgan, which is similar to some studies. The results could be justified by complete vaccination of the patients. Key words: Occult Hepatitis B, Hemodialysis, HBsAg, Gorgan
