Showing 6 results for Candidiasis
Nasrollahi Omran A(phd), Vakili L(msc), Jafarpur M(phd),
Volume 5, Issue 1 (4-2011)
Abstract
Abstract Background and objectives: Genital tract infections are among the most common causes of patients referred to therapeutic centers. Nearly 75% of women suffer from genital Candida infection, at least once in their lifetime. The aim of present study was detection of Candida species causing vaginitis and the evaluation of antimycotic effects of ketoconazol, clotrimazole and fluconazole against Candida species. Material and Methods: In this study, 210 vaginal samples were obtained from the patients suspected of Vaginal Candidiasis. Direct examination and culture were carried out for all specimens to detect the yeast. The isolated yeast species were then identified, using various different tests such as culture on corn meal agar, tween-80, germ tube test, and assimilation test by API 20C kit by using Sabouraud Dextrose Agar and microdilution broth, MIC90 and MIC50 of drug were measured and determined their drug resistance. Results: In the present study, 100 yeast colonies were isolated from patients %80 are C. albicans and the rest are C. parapsilosis(2%), C. tropicalis(6%), C. glabrata(4%), C. krusei(2%), C. guilliermondii (3%), C.stellatoidea(3%). In terms of drug resistance test MIC50 and MIC90 of fluconazole for candida albicans are 5.33 and 35.27μg/ ml, respectively, and for non-albicans candida are 3 and 21.4μg/ml, respectively. Clotrimazole MIC for Candida albicans (MIC50, MIC90) 0.97 and 4.9μg/ml, respectively, and for non-albicans 0.63 and 3.4/ml, respectively. Kectoconazole MIC for Candida albicans 2.43 and 16.45μg/ml, respectively, and for non-albicans 1.12 and 6.6μg/ml, respectively. Conclusion: Clotrimazole has been better than the two other drugs for Candida species on the whole, non albicans species are more sensitive than albicans species in the presence of the drugs used in this study. Key words: Candida, vaginal candidiasis, Resistance drug , Tonekabon.
Nasrollahi Omran, A, Nazemi, A, Kihanian, Sh, Aryana , N,
Volume 8, Issue 5 (1-2015)
Abstract
Abstract Background and Objective: With the development of drug resistance in strains of fungi, there is a considerable resistance of Candida albicans strains to fluconazole. Molecular studies are developing to determine the relationship of such a drug resistance with the increased gene expression of enzymes produced in drug-resistant Candida isolates. We aimed to evaluate the relationship between extracellular lipase gene (LIP8) expression of Candida albicans isolated from candidiasis and sensitivity or resistance to fluconazole. Material and Methods: Drug susceptibility of Candida albicans was performed in oral and vaginal candidiasis to determine the proportion of strains sensitive or resistant to fluconazole using NCCLS method. To evaluate and compare the expression of these genes in the susceptible and resistant strains, RT real-time PCR reaction was used. Results: Of 46 Candida albicans, 20 were susceptible, 12 were semi-susceptible and 14 were resistant to fluconazole. By using PCR reaction, the results showed that the expression of this gene in fluconazole-susceptible isolates was moderate, while it was high in the isolates resistant to fluconazole. Conclusion: The results of lipase gene (LIP8) expression showed that the additional expression of some genes of the enzymes responsible for virulence of Candida may also play a role in resistance to fluconazole. Keywords: Candidiasis, Lipase Gene Expression, RT real-time PCR, Fluconazole
Ebrahimzad, Mousavi Moh, Jamshidi Ali, Mohammadzadeh Rostami F (msc),
Volume 9, Issue 2 (7-2015)
Abstract
Background and Objective: Oral candidiasis is a common opportunistic infection in diabetic patients. Candida albicans is colonized on different oral surfaces such as tongue, palate, dental caries and plaques. This study aimed to determine the prevalence of Candida albicans in the mouth of diabetic patient.
Material and Methods: This cross - sectional study was conducted on 120 diabetic patients. After completing the questionnaire, the saliva samples were obtained and cultured on sabouraud agar medium containing 0.005% chloramphenicol. For final diagnosis, Germ tube test and Sugar absorption test (with API 120 kit) were used.
Results: Of 120 patients, 61 (50.8%) were positive for Candida. Candida infection rate was relatively higher in older patients and in women. The rate was significant in the patients using dentures (P< 0.001) and the ones keeping on smoking (P <0.038). The infection in the patients with type II diabetes was higher than the cases with Type I.
Conclusion: Given the high prevalence of Candidiasis and Candida colonization in the mouth of diabetic patients, it is a necessity to identify and treat these infections in order to prevent further complications.
Keywords: Candidiasis, Diabetes Mellitus, Fungal Infections
Azadeh Abedzadeh Hajar, Mohammad Dakhili, Mojgan Saghazadeh, Seyyed Soheil Aghaei, Razieh Nazari,
Volume 14, Issue 3 (5-2020)
Abstract
Background and Objective: The prevalence of the infections caused by Candida species has led to a significant increase in their resistance to antifungal compounds. The aim of this study was to i) investigate drug resistance ii) evaluate the incidence of Candida albicans drug resistance pattern in the vaginal samples of women referring to health centers of Qom province, and iii) examine the effect of Zn nanoparticles combined with fluconazole against C. albicans isolates.
Methods: This experimental, descriptive study was performed on 120 patients of candidiasis. In order to identify Candida albicans, direct experiments, differential culture, Germ tube test and sugar assimilation test (API20C kit) were conducted. The effect of different antifungal drugs and zinc nanoparticles and the synergistic effect of fluconazole with zinc nanoparticles were investigated by disk diffusion method. Minimum Inhibitory Concentration (MICs) of all cases was further specified.
Results: Of the 120 samples, 50 (41.6%) were identified as Candida albicans. These strains were resistant to certain antifungal drugs while others were semi-sensitive and sensitive. The lowest and the highest mean diameter of inhibition zone in all Candida albicans isolates belonged to ketoconazole (15.64 mm) and fluconazole nano-ZnO (26.76mm), respectively. The lowest and the highest MICs were observed in fluconazole- nano-ZnO and nano-ZnO, respectively.
Conclusion: The synergistic effect of Zn nanoparticles with fluconazole can be conducive to the treatment of vaginal candidiasis.
Raheleh Alhashem, Seyededeh Sedigheh Hosseini,
Volume 18, Issue 1 (1-2024)
Abstract
Background: Vulvovaginal candidiasis (VVC) remains one of the most common infections of the female genital tract. This local study was conducted to identify the spectrum of Candida species associated with VVC and recurrent vulvovaginal candidiasis (RVVC) using different phenotypic and genotypic methods and assess their fluconazole susceptibility.
Methods: High vaginal swabs were collected from 576 patients with VVC. Swabs were subjected to KOH 10% and cultured on Sabouraud dextrose agar. The species identification of Candida isolates was made using phenotypic methods, including the germ tube test, corn meal agar (CMA), CHROMagar Candida, and PCR-RFLP as the best genotypic methods for confirmation of Candida species in vulvovaginal candidiasis. Fluconazole susceptibility testing was performed using the disk diffusion method.
Results: The data were analyzed using the chi-square test. The prevalence of candidiasis was 61%. The socio-demographic profile corresponded to a woman aged 29.14 ± 4.8 years on average, pregnant (77%), diabetic (19%), with hypertension (10%), recurrent (9%), a history of fluconazole use (4%), and under 30 years of age (73%).
Vaginal swab cultures yielded Candida growth in 352 cases (61%). Candida albicans was the predominant isolated species (78%), while the most common non-albicans species were C. glabrata (14.7%), C. parapsilosis (4.2%), C. tropicalis (2.8%), and C. krusei (0.56%). Isolates of C. glabrata and C. krusei exhibited the highest rate of DDS resistance among all isolates tested.
Conclusion: C. albicans was the most common species. Careful periodical surveillance is needed to identify any changes in the susceptibility patterns to fluconazole with the increased use of this antifungal agent in Sayad Shirazi tertiary care hospital, Iran.
Dr Pius Omoruyi Omosigho, Miss Guobadia Precious Oghogho, Ms Ugiagbe Victory Osayekewmen, Mr Olalekan John Okesanya, Dr Janet Mosunmola Oladejo, Dr Uyigue Paulinus Osarodion,
Volume 19, Issue 2 (3-2025)
Abstract
Background: Candida species are known to be the most frequently encountered fungal pathogens in humans. There has been a noticeable rise in the occurrence of human infections caused by Candida over the past few decades. This cross-sectional study aimed to characterize the epidemiology and antifungal susceptibility patterns of Candida species isolated from clinical specimens in a tertiary hospital in Benin, Edo state, Nigeria.
Methods: A total of 104 Candida isolates were obtained from various clinical specimens using a simple random sampling technique. The isolates were cultured on Chromagar Candida and identified based on characteristic color production. Antifungal susceptibility testing was performed using the disk diffusion method for Amphotericin B, ketoconazole, fluconazole, and Nystatin. Statistical analysis was conducted using SPSS version 20.0.
Results: Candida albicans was the most prevalent species, accounting for 72.1% of isolates, followed by Candida krusei (17.3%), Candida tropicalis (7.7%), and Candida glabrata (2.9%). High vaginal swabs showed the highest frequency of Candida isolates (46.2%). Females exhibited a higher preponderance of candidiasis (81.3%) compared to males. Candida albicans showed significant resistance to Amphotericin B and fluconazole, while other species demonstrated mixed susceptibility patterns. The antifungal susceptibility testing indicated variations in resistance patterns among different Candida species.
Conclusion: The study revealed a predominance of Candida albicans in clinical specimens, with emerging cases of non-albicans species. Antifungal resistance to clinically available agents raised concerns, necessitating continuous surveillance and monitoring of susceptibility patterns. The results underscore the importance of developing targeted strategies to combat the
