Bachground:Carbapanenems are β-lactam antibiotics that have multiple bactericidal activities. These antibacterial are usually considered to be the last-choice drugs in treating infections with multi-drug resistant Gram-negative bacteria. Carbapenemase producing organisms (CPOs) are associated with serious health care-associated infections, making the mortality rate higher.
Methods:250 samples were collected with sterile cotton swabs; from wounds and burn wounds patients (140 wound swabs and 110 burn swabs). These swabs were cultured on MacConkey agar and cetrimide agar. Cultures were incubated at 37°C for 18–24 hrs. The appearance of the colonies was evaluated by colour, shape, and overall morphology. BD Phoenix™ M50 device is used in identifying and making sensitivity test with BD RAPIDEC® CARBA NP assay in detecting carbapenemase enzymes.
Results:27(38.02%) isolates were carbapenemase producing and multi-drug resistance , distributed as following : 12 Carbapenemase producing Pseusomona aeruginosa multi-drug resistance, 9Carbapenemase producing Escherichia coli multi-drug resistance, 5carbapenemase producing Enterobacter cloacae multi-drug resistance and one isolate of Carbapenemase producing Klebsiella pneumoniae multi-drug resistance. The current study reported CP-Pseudomonas aeruginosa and CP-Klebsiella pneumoniae isolates as pathogens with the highest number of antibiotics resistance (resist 15 different antibiotics from seven classes of antibiotics).
Conclusion: In current study ,the carbapenemase producing organisms (CPOs) were highly MDR against most classes of antibiotics due to misuse or overuse of antibiotics,
one of the most urging topics to follow is to recognize and develop modern drugs to combat the carbapenem resistant organisms with multi-drug patterns that are known to cause higher morbidity and mortality among patients globally.
