Leila Pirdel, Maryam Safajoo, Masoud Maleki,
Volume 19, Issue 5 (9-2025)
Abstract
ABSTARCT
Background and objectives: Mesenchymal stem cells (MSCs) are well known as a major immune modulator. A subgroup of the nucleotide-binding oligomerization domain (NOD) like receptors (NLRs) has been recently found to play an immune/inflammatory regulatory role.
We aimed to analyze and compare the gene expression level of the NOD‐like receptor family pyrin domain containing protein (NLRP), such as NLRP6 and NLRP12, in Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) treated with interferon-gamma (IFN-γ), the pro-inflammatory cytokine, and untreated cells as well.
Methods: The immunophenotypic characterization of the isolated WJ-MSCs was performed by flow cytometry. Next, they were cultured with or without IFN-g, followed by the comparison of expression level of NLRP6 and NLRP12 genes by using qPCR.
Results: The treatment of cells with IFN-γ indicated a statistically significant increased expression of NLRP12 gene as compared to untreated cells while the expression of NLRP6 was failed to detect in the cells with or without IFN-γ treatment.
Conclusion: The altered expression level of NLRP12 might be suggested its contributory role in the inflammatory regulation mediated by WJ-MSCs in response to the exposure to IFN-g; however, additional studies are needed to validate its role in experimental inflammatory-related disease models.
