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Showing 7 results for Probiotic
Frequency of Lactobacillus Strains in Foods of East Azerbaijan Cities, Iran
M Bonyadi, M Rahimi, M Nahai, M Akbari Dibavar, F Mirzaee,
Volume 4, Issue 2 (10-2010)
Abstract

Abstract

Bachground and objectives:

micro-organisms, usually made from strains of the genera

Probiotics are nonpathogenic and beneficialLactobacillus and

Bifidobacterium. The probiotics are known as dietary supplements withLactobacillus and

Bifidobacterium , in sufficient quantities and specific compound, are used for

Material and Methods:

obtained from dairy products, yogurt, grains cheese, saffron flower, fresh

pinto beans, red beans, fresh green beans . All samples were incubated in

MRS Agar cultures at 42, 37, and 25

isolated, concentrated, and lyophilized. Finally the differential recognition

was performed in deferential cultures.

In this cross-sectional study 100 Samples were°c for two days, and then bacteria were

Results

isolated. Lactobacillus delbrueki and L.bulgaris(each 10 cases)

(8 cases) and L .casei (5 cases) are the most prevalent Lactobacillus strains.

Lactococcus themrophilus the most frequent Lactococcus where isolated in

this study.

: Of 100 food samples 19 Lactobacillus and Lactococcus strains were، L.salivarius

Conclusion:

especially Lactobacillus strains are presented in foods. It is recommended that

these probiotics be isolated and proliferated and used in industry and also for

therapeutic purposes.

Based on the results of this study, many kinds of probiotics

Key words:

probiotic, Lactobacillus, foods

therapeutic purposes and food industry. The aim of this study was to evaluate

the frequency of Lactobacillus and Lactococcus strains in food stuffs cities in

Eastern Azerbaijan, Iran.

beneficial effects on consumer health. In some countries,


Antimicrobial Effect of Lactic Acid Bacteria against Common Pathogenic Bacteria
Mohammad Mohammaddoost Chakoosari, Mohammad Faezi Ghasemi, Alireza Masiha, Reza Kazemi Darsanaki, Abolfazl Amini,
Volume 9, Issue 5 (11-2015)
Abstract

Abstract

      Background and Objective: Probiotics are living microorganisms that have beneficial effects on the health of digestive system. The aim of this study was to evaluate the antimicrobial ability of acidic and neutral supernatants (culture supernatant) of lactic acid bacteria against common bacterial pathogens.

       Methods: Four species of lactic acid bacteria (Lactobacillus plantarum PTCC1745, Lactobacillus PTCC1608, Lactobacillus Saki PTCC1712 and Lactobacillus Lactis PTCC1336) were obtained from the microbial collection of Iranian Research Organization for Science and Technology in Lyophilized form. The antimicrobial activity of neutral and acidic supernatants against bacterial pathogens was investigated using the Disk and Well Diffusion Agar methods.

      Results: Lactic acid bacteria showed good antimicrobial ability against six pathogenic bacteria with the highest inhibitory effect observed in Lactococcus lactis against E. coli PTCC1399 through well method with an average diameter of 14 mm inhibition zone. In this study, the well diffusion method was far more sensitive compared to the disk method and acidic supernatants showed higher antimicrobial efficiency compared to neutral types.

      Conclusion:  the Metabolites produced by lactic acid bacteria are able to inhibit the growth of pathogenic bacteria that can be an important and practical solution for the prevention and treatment of infections and ultimately improve human health.


Effects of Lactobacillus casei Culture Supernatant on Differentiation of K562 Cell Line
Kazem Maftuni , Peyman Zare ,
Volume 11, Issue 5 (9-2017)
Abstract
ABSTRACT
           Background and objective: Considering the toxic side effects of chemotherapy in treatment of cancer, anticancer drugs of natural origin including probiotic Lactobacillus strains have recently attracted a lot of attention.
Methods: After culturing chronic myeloid leukemia cell line K562 in 96-well plates, effects of different concentrations of culture supernatant from Lactobacillus casei on differentiation of the cells were investigated after 48 and 72 hours under an inverted microscope. Number of live cells and percentage of viable cells were determined by trypan blue exclusion test of cell viability. Cytotoxicity was assessed by MTT assay. Data analysis was performed by SPSS (version) 22 using one-way analysis of variance and Tukey's test at significance level of 0.05.
          Results: Secondary metabolites from the probiotic bacteria L. casei induced cellular differentiation, exerted anti-cancer effects and inhibited growth in K562 cells. Apoptotic cell death was confirmed by MTT and DNA fragmentation assays in a way that increasing the dilution from 1.2 to 1.32 significantly increased the viability of cells (P=0.001). In addition, increasing the dilution significantly increased the number of live cells in the first 48 hours (P=0.001).
        Conclusion: Culture supernatant of L. casei reduces the number of live cells, and induces apoptosis and monocytic differentiation in K562 cells in a dose- and time-dependent manner. Therefore, combined chemotherapy and differentiation therapy using such supernatants could be useful for treatment of cancer.
        Keywords: Cell differentiation, K562 cell line, Probiotic, Lactobacillus casei.
Inhibitory Effect of Probiotic Bacteria Isolated from Camel Milk on Clinical Strains of Drug-Resistant Helicobacter pylori
Afsaneh Sikarchi , Leila Fozouni ,
Volume 12, Issue 2 (3-2018)
Abstract
 
ABSTRACT
           Background and Objectives: Helicobacter pylori is the most common cause of gastritis and ulcer worldwide. Treatment of such infections may lead to failure due to drug resistance. This study aimed to investigate the antimicrobial effects of bacteria present in camel milk on the growth of drug-resistant clinical isolates of H. pylori.
           Methods: In this cross-sectional study, biopsy samples from 75 patients with digestive symptoms were transferred to laboratory in transport medium containing homogeneous compounds. In order to isolate H. pylori, urease-positive biopsies were promptly cultured in brucella agar enriched with defibrinated sheep blood and fetal calf serum. Disk diffusion agar test was used to evaluate antibiotic susceptibility and agar well diffusion method was applied to study the antagonistic effect of probiotics isolated from camel milk on the H. pylori isolates.
           Results: The frequency of H. pylori isolates was 42.7%. The highest rate of resistance was observed against metronidazole (56.3%). In addition, the rate of resistant to amoxicillin, ciprofloxacin, and clarithromycin and tetracycline was 31.3%, 18.8%, 15.6%, respectively. Lactobacillus plantarum (59.3%) was more frequent than other Lactobacillus species. L. plantarum, Lactobacillus fermentum and Lactobacillus casei showed favorable inhibitory effects against the H. pylori isolates, but L. plantarum (with inhibition zone diameter of 20.3 mm) showed the highest inhibitory effect.
           Conclusion: Considering the increasing rate of drug resistance and the inhibitory effect of probiotics isolated from milk, health providers recommend that promoting consumption of probiotic food seems beneficial for the general population and those suffering from gastrointestinal disorders.
           Keywords: Helicobacter pylori, Drug resistance, Camel, milk, Probiotics.

Positive Effects of Spray-Dried Streptococcus thermophilus on Healing of Second-Degree Burn Wounds in Wistar Rats
Amir Abbas Barzegari, Masood Hashemzaei, Ali Reza Aslihematti,
Volume 13, Issue 2 (3-2019)
Abstract
ABSTRACT
              Background and Objectives: Current medications used for the treatment of burn wounds have some side effects and disadvantages. Oral or topical administration of probiotic bacteria is thought to be useful for treatment of skin disorders. The aim of this study was to evaluate effects of spray-dried Streptococcus thermophilus on healing of second-degree burn wounds.
              Methods: After induction of second-degree burn on back of 80 male Wistar rats, the animals were randomly allocated to negative control (no treatment), first vehicle control (treatment with Eucerin), second vehicle control (treatment with skim milk) and experimental (treatment with bacterial ointment) groups. Wound healing rate (percent) and histopathological parameters of wound samples were evaluated on post-burn days 1, 3, 7 and 14.
              Results: On days 3 and 7, macroscopic results showed that the healing rate was significantly higher in the experimental group compared to the control groups. Histopathological analysis of wound samples showed increased fibroblastic migration, collagen formation and re-epithelialization in the wounds treated with probiotic bacteria compared with the wounds of control groups.
              Conclusion: Our results indicate that the topical use of spray-dried S. thermophilus could be useful for the treatment of burn wounds.
              Keywords: Streptococcus thermophilus; Probiotics; Burns; Wound Healing; Rats.

Antibacterial Effects of Compound Bifilact on E.coli and Campylobacter jejuni
Hamidreza Ebrahimnezhad, Leila Barzegar, Davoud Esmaeili,
Volume 14, Issue 1 (1-2020)
Abstract
ABSTRACT
           Background and Objectives: Probiotics are live microorganisms that function through  various mechanisms  and affect the alteration of the commensal microbiota against pathogens. Nowadays,  given the problems associated with antibiotics use, probiotic strains  offer a novel and  appropriate  alternative for the treatment of diseases such as diarrhea. The aim of this study was to investigate the antibacterial synergism of Lactobacillus spp., Bifidobacterium spp. and Escherichia coli strain Nissle 1917 (ECN) on the clinical sample of diarrheagenic E.coli and Campylobacter jejuni.
           Methods: A paper disk-diffusion technique was used to evaluate the antibacterial activity. Sterile 6 mm paper disks were saturated with probiotic suspensions made by settling probiotic medications into distilled water. Three kinds of disk were prepared. One disk was prepared for Lactobacillus spp. and Bifidobacterium spp., another for ECN, and the third was made by combined probiotics. Clinical samples of diarrheagenic E.coli and Campylobacter jejuni were cultivated on Muller Hinton agars, and disks were placed on the inoculated Muller Hinton agars. All plates were incubated under microaerophilic and appropriate conditions.
           Results: The zone of inhibition (ZOI) of the bacterial growth was measured. All pathogenic microorganisms showed sensitivity to the probiotic disks. The combined disks had better effects against pathogens compared with single disks.
           Conclusion: A considerable synergistic effect was observed in the results of combined probiotics; therefore, combined strains can be more efficient against intestinal pathogens in comparison with single probiotics.
           Keywords: Probiotic, Lactobacillus, Bifidobacterium, Escherichia coli Nissle, Diarrhea, Campylobacter jejun.i.

Effects of kefir on liver function tests and histopathological changes in rats exposed to aflatoxin B1
Seyed Ahmad Sajjadi, Zahra Moosavi, Farhad Niknejad, Abdollah Jamshidi,
Volume 17, Issue 4 (7-2023)
Abstract
Seyed ahmad Sajjadi1 , Zahra Moosavi2 , Farhad Niknejad3 , Abdollah Jamshidi 4
Background: Aflatoxin B1 (AFB1) is one of the most important mycotoxins that contaminate food worldwide. Long-term consumption of foods contaminated with AFB1 endangers human health. Detoxification of AFB1 from food improves community health. A Specific approach to aflatoxin reduction is the use of probiotics. Kefir drink is a strong probiotic. The purpose of this study was to investigate the protective effect of kefir drink on AFB1-induced hepatic injury in adult male rats
Methods: In this experimental study, 24 adult rats weighing between 150 and 200 g were used. The rats were randomly divided into 4 groups: 1) control, 2) AFB1 (50 μg/kg body weight), 3) kefir drink (10 mL/kg body weight), and 4) AFB1 + kefir drink. Aflatoxin and kefir drink received through oral gavage. At the end of the experiment (8 weeks), blood and liver samples were collected for different assays. Liver function tests and histopathological examinations were performed. Data were analyzed using 1-way analysis of variance (ANOVA) and at a significance level of <0.05.
Results: Aflatoxin B1 significantly increased serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (T.Bili), as well as decreased total protein (T.P) content, compared to the control group (P < 0.05). Aflatoxin B1 induced histological changes in the liver. The results obtained from the groups treated with kefir drink with and without AFB1 were not significantly different from the control group. Histopathological changes were not found in groups treated with kefir drink with and without AFB1.
Conclusion: The consumption of kefir drink reduced AFB1-induced disruptions in rats’ livers.
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