Showing 49 results for Ros
K Ghazisaidi, F Fateminasab, Sh Vatani, Y Azimi, S Bakhshandeh Nosrat, M Mohamadi,
Volume 2, Issue 1 (4-2008)
Abstract
Abstract Background and objectives: Ureaplasma urealyticum is one of the most important causes of Nongonococcal urethritis (NGU) and non-specific urethritis (NSU) in men. Also Mycoplasma hominis has a role in causing NGU and NSU. The aim of this study was the detection of Mycoplasma hominis & Ureaplasma urealyticum in men suffering from NGU and NSU by prostatic massage method and first- void Urine samples. Material and Methods: Urethral secretion samples, taken after prostatic massage and first-void urine, of 75 patients referred to department of bacteriology of Tehran University of Medical Sciences were examined for ureaplasma and mycoplasma by culture method in 2004-2005 Results: In prostatic samples, 19 and 11 samples are positive for ureaplasma and Mycoplasma, retrospectively. In first-void urine samples, 17 and 9 samples are positive for ureaplasma and Mycoplasma, retrospectively. Conclusion: The results show that if it is impossible to obtain urethral sample via prostatic massage , we can use first- void urine instead. Key words:Mycoplasma hominis, Ureaplasma urealyticum,Nongonococcal urethritis, Nonspecific urethritis, prostatic massage, first voided Urine
F Tohidi, M Qorbani,
Volume 2, Issue 2 (10-2008)
Abstract
Abstract Background and Objectives: one of the endemic foci for Cutaneous Leishmaniasis in Iran is Mashhad in which limited outbreak have recently been reported. The commonly used method for diagnosis is the clinical features confirmed by direct microspic examination and culture or biopsy. We compared these two tests to determine the level of their sensitivity, specificity and positive predictive value. Material and Methods: we performed this comparative-analytic study on 73 patients suspected of having ulcers Leishmaniasis in Mashhad, Iran. Giemsa was staining the smears and the samples cultivated on Di-phasic N.N.N. culture media . Analysis was performed by SPSS version 11.5 and Chi square test. A P- value less than 0.05 were considered as a significant. Results: In 43 cases (58.9%), both the smear and culture are Positive. In 13 cases (17.8%), the smear is negative but the culture Positive. In 17 cases (23.2%), both smear and culture are negative. The two methods are positively correlated (82%). Sensitivity, Specifity, Positive predictive Value and negative predictive value are 76.7%, 100%, 100% and 56.7%., respectively. Conclusion: when the smear is positive, there is no need for culture. However, the opposite is not true. Key words: Cutaneous Leishmaniasis, Laboratory Diagnostic, Direct Microspic Examination, Culture.
Z Shahandeh, F Sedighian, Mt Salehi Omran, F Saberian,
Volume 3, Issue 1 (4-2009)
Abstract
Abstract Background and objectives: Coronary Artery Disease is one of the most important causes of death in the world. Atherosclerosis is a complex disorder and many factors cause it . In recent years , a relationship between infectious agents, particularly Chlamydia pneumonia, and atherosclerosis was found. This research was aimed at serological studying of Chlamydia pneumonia in the coronary artery patients and healthy ones. Material and Methods: We carried out this cross-Sectional study on 102 patients with CAD and 142 individuals without any history of CAD. The subjects were admitted to Dr. Beheshty hospital, Babol, from July 2005 to September 2006. The subjects’ serums were collected to detect specific anti Chlamydia pneumonia antibodies (IgA and IgG), using ELISA method. Data were analyzed by chi-square, using SPSS software. Results: The mean age of patients and healthy subjects were 60.6 and 43.7 years, respectively. Fifty-nine point eight percent of cases and 40.8% of healthy subjects are men. The results show that 45.1% of patients and 47.9% of healthy subjects have positive titer of IgG and negative titer of IgA (chronic). There is no significant correlation between patient and healthy groups (P=O.698). Also 88.2% of patients and 81% of healthy group have positive titer of anti Chlamydia pneumonia antibody (IgG). Conclusion: In spite of high titer of IgG, there is no significant relationship between Chlamydia pneumonia and atherosclerosis. It seems that further experiments are necessary to prove the relationship between Chlamydia and atherosclerosis. Key words: Atherosclerosis, Chlamydia pneumonia, IgA, IgG
K Dorry, R Nedaeinia, V Hemayatkhah Jahromie, M Naderian,
Volume 4, Issue 1 (4-2010)
Abstract
71/Spring summer 2010, Vol.4, No.1 Medical Laboratory Journal The Effects of Anabolic-Androgenic Steroid Drugs Consumption on Blood Factors in Bodybuilder’s Athletes in Jahrom,Iran Abstract Background and objectives: Mass media and the press present documentary reports of taking illegal energetic drugs by athletes who are tempted to enhance their performance. The present study aims at determinrg the effect of two illegal drugs on the athletes, blood factors. Material and Methods: The participants are 20 volunteer athletes selected unrandomly, 10 as a control and 10 as an experimental group. First, their blood samples were obtained from the medial cubital vein of left hand and then they were subjected to body building exercises for six weeks. The control group did not take any drugs while, the experimental one took oxymetholone steroids and methandrostenolone orally, based on pre – determined schedule during the exercises. During this period, Venipuncture was carried out for five times and the effects of drug on the blood factors were investigated. Finally, we analyses the findings by statistical Tests. Results: The results show that the levels of HCT (51.1 % vs. 46%), Hb (16.6 g/dl vs.15.6g/dl) and Reticuloeyte (0.9% vs. 0.5%) are increased significantly (P<0.05). there is no meaningful differences in the other blood factors such a WBC, MCV, MCHC an MCH. Conclusion:Based on this, findings, taking oxymetholone and methandrostenolone, which have prominent effects on the body functions, result in major changes in some blood factors such Hb, Hct and Retic. Key Words: Oxymetholone, Methandrostenolone, blood factors Dorry K (MSc) Instructor ofMicrobiology, Islamic Azad University, Jahrom Branch Nedaeinia R (MSc) Microbiology, Undersecretary for Food and Drug, Isfahan University of Medical Sciences Hemayatkhah JahromieV (PhD) Instructor ofMicrobiology, Islamic Azad University, Jahrom Branch NaderianM (PhD) Assistant Professor of Physical education, Isfahan University Corresponding: Dorry K Email: s.dorry@gmail.com
H Rafieemehr,
Volume 4, Issue 2 (10-2010)
Abstract
Abstract Bachground and objectives: Higher than needed blood orders not only adversely affect blood quality but also impose extra expenses on treatment center and patients. We aimed at determining the frequency of packed red blood cell transfusion in Besat hospital of Hamadan in 2009-2010. Material and Methods: This was a descriptive study on 926 blood order forms in Besat hospital. The data were collected using forms, in Hamedan teaching hospital (Besat) from March 2009 to March 2010.The amount of blood order, blood consumption, crossmatch /transfusion ratio(C/T) and transfusion index (TI) in different wards of hospital. Results: Out of 926 patients aged averagely 28 years, 37%were females and 63% are males. The overall ratio of C/T and TI are 2.44 and 0/63, respectively which are considered to be optimal in comparison with the standard figures of C/T<2.5 and TI≥0.5.The results show that the highest C/T is in surgery ward and the lowest in burn ward, but the highest TI is related to Hematology ward and the lowest to urology ward. Conclusion: Packed cell consumption, C/T Ratio and TI in Besat hospitals is normal but not ideal. It seems that absence of hospital blood transfusion committees and lack of active contribution of physicians, are the major obstacles in reforming blood utilization. Key words: Packed cell, Crossmatch, Transfusion Index
M Rahbani-Nobar, Mh Somi, A Fattahi, N Dolatkhah, M Nourazarian, S J Seyedi-Khoshknab, B Pourasghari, H Dolatkhah,
Volume 4, Issue 2 (10-2010)
Abstract
Abstract Bachground and objectives: Epidemiological studies have shown that using tobacco products is one of the main factors in forming malignancies in various tissues of the body. There is more than 600 μgr nitric oxide radical (NO°) in gas phase in each cigarette with fresh smoke. Hence, oxidation of nitrogen components in tobacco, more than 100 μgr of atmospheric NO°is produced by smoking, would be transferred to the body without any filtration. We studied nitric oxide levels in the gastric juice of smokers and non-smokers patients with active peptic ulcer. Material and Methods: In this study, 43 smoker patients with active peptic ulcer (14 female & 29 male) referred to gastroenterology clinic with mean age of 45.30±13.16 as case group.Forthy-three non-smokers without peptic ulcer (13 female & 30 male) aged 42.67±16.04, 43 smoker without peptic ulcer (16 female & 27 male) with mean age of 44.58±12.07 and 43 non-smoker with active peptic ulcer (20 female & 23 male) with mean age of 45.37±13.39 were selected as control groups of 1, 2 and 3 ,respectively. The level of Nitric oxide in gastric juice was measured by using Greiss colorimetric method. Results: Comparing with control group one and two, meaningful rise is noticed in mean level of nitric oxide case group (p<0.0001). Mean levels of NO in control group 1, 3 and case group are 4.21±1.13, 5.37±2.26, 7.90±2.12 μmol/L, respectively. Nitric oxide level in case group in comparison with control group 2 dose not show Significant difference (p=0.656). Mean levels of NO in control 2 and case groups are 7.45±1.54 and 7.90±2.12 μmol/L, respectively. Conclusion: It can be concluded that cigarette smoking may be one of the cause of increased level of gastric juice nitric oxide. This increase may be due to component in cigarette smoke and tar. These components can cause DNA damage through oxidation-reduction cycle and consequently increase the risk of malignancies in gastric tissues. Key words: Cigarette Smoking, Nitric Oxide, Nitrosative Stress, Active Peptic Ulcer
Moradi Av, Azadfar S, Fatemehcheraghali, Javid N, Ghaemi A, Tabarraei A,
Volume 5, Issue 2 (10-2011)
Abstract
Abstract Background and objectives: Mumps virus is one of the first known causative agents of meningitis in children. On-time diagnosis is the first step in treating meningitis. We aimed to evaluate Mumps virus meningitis in children in Gorgan, Iran Material and Methods: CSF and blood samples were taken from children with meningitis, Jun 2008 till Sep 2010. For 40 samples with negative bacterial culture, Extraction of viral RNA was carried out and Real-time PCR was performed for detection of Mumps virus. Demographic, clinical, biochemical and cytological data were collected. We run SPSS version 18 to analyze the data, using Chi Square (p<0.05). Results: three (7.5 %) samples have Mumps virus, two boys and one girl. All three positive cases have 0.5-1 degrees Celsius fever and vomiting but no bulging fontanel. They have not Kernig, Rodor, Brudzinski’s sign, hepatosplenomegaly, lymphadenopathy, pharyngitis and rash. ESR is higher than normal in all positive cases and CRP is positive in two cases. Protein of CSF in one case is higher than normal range. Conclusion: meningitis is an emergency condition therefore, molecular diagnostic techniques are recommended for early diagnosis and intervention. Key words: meningitis, mumps virus, cerebrospinal fluid, Real-Time PCR
Soltan Dallal Mm, Rahimi Forushani A, Bakhtiari R,
Volume 6, Issue 1 (4-2012)
Abstract
Abstract Background and objectives: Helicobacter pylori is a helical gram negative bacterium with polar flagella, discovered by Warren and Marshall in 1983. Helicobacter pylori exist in the stomach mucus tissue of less than 20% of people under 30 years old, but this amount would increase up to 40% and 60% in 60- year- old people. The aim of this study was to compare three methods of culture media, direct slide staining and the urease test for the rapid diagnosis of bacterium in case of peptic or duodenal ulcer. Material and Methods: In This descriptive study, duplicate biopsy specimens were taken from 82 clients referring to four different Hospitals .In endoscopy room of the Hospitals, a rapid urease test were carried out on one of duplicate specimens for the presence or non-presence of Helicobacter pylori. In order to see the Helicobacter pylori in the tissues, three slides using foushin, giemsa, and gram staining were prepared from the second specimens. Then, the specimens were incubated into selective culture media and incubated for 4-6 days in micoraerophilic condition. Results: Of 82 tested specimens 70(85.5%) and 66(80.5%) are identified as Helicobacter pylori by positive urease and culture medium, respectively. The frequency of foushin, giemsa, and gram staining are 67 (81.7%), 66 (80.5%), and 61 (74.4%), respectively. The foushin staining is the best with 100% sensitivity among the other methods. Conclusion: Based on difference between proportions, There is no significant difference between staining methods (foshin, giemsa, gram staining) and culture media in all cases. Key words: Helicobacter pylori, microscopic methods, urease test, culture media, identification
Khoshdel Rad N, Mashayekhi F, Mirzajani E,
Volume 6, Issue 1 (4-2012)
Abstract
Abstract Background and objectives: C-Met is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor (HGFR). The HGF receptor possesses tyrosine -kinase activity and it is essential for embryonic development, wound healing and cancer. Many proteins are proteolytically released from the surface by a process known as ectodomain shedding. Shedding occurs under normal physiologic conditions and can be increased in certain pathologies. C-Met can be seen among many receptors for which ectodomain shedding has been shown. The aim of this study was to determine the concentration of soluble c-Met in the cerebrospinal fluid (CSF) and serum samples of patients with viral and bacterial meningitis. Material and Methods: in this study, 75 CSF and serum samples of patients with bacterial meningitis, 71 with viral meningitis and 82 normal controls were investigated. The soluble c-Met concentration was determined by enzyme linked immunosorbent assay (ELISA). Result: the amount of soluble c-met in CSF of patients with bacterial meningitis ( 83.91±5.50), viral meningitis ( 80.41±4.71) and control group ( 22.66±3.39) are compared with that in serum of patients with bacterial meningitis ( 561.58±25.87), viral meningitis ( 550.50 ±34.34) and control group ( 256.25±18.55). There is significant increase in the CSF and serum’s soluble c-Met expression in the patients with meningitis, in comparison with control group. Conclusion: The data presented here indicate that soluble c-Met is a constant component of human serum and CSF, but it can not be used for differentiating bacterial meningitis from viral meningitis. Key words: Soluble c-Met, concentration, cerebrospinal fluid, serum, meningitis
F Mashayekhi, F Rajaei,
Volume 6, Issue 2 (10-2012)
Abstract
Abstract
Background and objectives: Meningitis is one of the most common infectious of the central nervous system (CNS), defined as an inflammation of the meninges. LIF is a potent pro-inflammatory factor. Cerebrospinal fluid (CSF) contains the growth factors and cytokines whose concentrations have been changed in most neurological diseases. The aim of this study was to determine the LIF concentration of serum and CSF in the children with bacterial meningitis.
Material and Methods: In this study, the total protein concentration (TPC) and LIF in the serum and CSF of normal subjects and children with bacterial meningitis were measured by enzyme linked immunosorbent assay (ELISA).
Results: the Values of serum TPC for children with meningitis (74.17±7.73 g/L) and controls (73.50±7.28 g/L) are not different significantly (P=0.7), and the TPC in the CSF of children suffering from meningitis and controls are 35±0.03 and 0.34±0.05 g/L, respectively (P=0.65). The concentration of serum LIF for children with meningitis( 253±19.14 ng/ml) is higher than that of controls (49.75±8.97 ng/ml), and also the concentration of LIF in the CSF of the children with meningitis (116.25±8.60 ng/ml ) is significantly higher than that of controls which is 9.04±1.83ng/ml (P<0.001).
Conclusion: The LIF concentration in the CSF and serum may provide additional information in the differential diagnosis of meningitis. It is also concluded that LIF could be significantly involved in the pathophysiology of meningitis.
Key words: Serum, Cerebrospinal fluid, Leukemia inhibitory factor, Children, Bacterial meningitis
Z Gharibi, F Daadras, M Maghsood, M Fallah,, M Saeedijam,
Volume 7, Issue 4 (1-2014)
Abstract
Abstract
Background and Objective: Microsporidia is an obligatory intracellular parasite known as an opportunistic infection in immunocompromised patients. Its laboratory diagnosis is relatively difficult and modified trichrome staining is a standard diagnostic method for detection of Microsporidia. The aim of present study was to identify intestinal Microsporidia in kidney- transplanted patients, using modified Trichrome Staining and Calcofluor White Methods.
Material and Methods: In 2012, 180 stool specimens were taken from kidney transplanted- patients given immunosuppressive drugs in Hamadan, Iran. To identify Microsporidia we use modified Trichrome Staining and Calcofluor White Methods.
Results: The mean duration of kidney transplant and immunosuppressive drug using was 5.5 Years. Only one female patient was positive for Microsporidia.
Conclusions: Owing to low frequency of this opportunistic infection among kidney transplanted patients, we can conclude that their hygienic conditions are good enough and they are not exposed with the parasites.
Key words:Microsporidia, Modified Trichrome Staining,Kidney Transplant, Calcofluor White, Hamadan
N Hashemi, Y Yazdani,
Volume 8, Issue 3 (8-2014)
Abstract
Abstract
Background and Objectives: Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease. Mucosal feeding of myelin basic protein binding to the cholera toxin B subunit can reduce the intensity of the immune response in MS patients. Expression system, the domain composition of the fusion protein, accessibility of two domains, codon adaptation index (CAI) and GC contents are very important for the large scale production of fusion protein.
Material and Methods: we used DNA2, PSIPRED and ProtParam softwares for designing the best form to produce fusion protein. Moreover, the correct open reading frame of myelin basic protein was also considered. First the coding sequence was verified and then synthesized. For confirmation of the recombinant vector, PCR test was carried out using T7 primers. Finally it was inserted into the cloning site of pET28 expression vector.
Results: After coding optimization, the CAI rate was increased from 64 % to 80% and GC content from 41 % to 49%. The presence of a band near 700bp resulted from PCR amplification test demonstrates the correct cloning of recombinant vectors in the cloning site of pET28 expression vector.
Conclusion: According to software and experimental analysis, the designed sequence probably in the best form could be used for production of recombinant protein.
Keywords: Multiple Sclerosis, Cholera Toxin, Myelin Basic Protein
E Abdollahi, F Tavasolian, M Samadi,
Volume 8, Issue 3 (8-2014)
Abstract
Abstract
Background and Objective: Atherosclerosis is an inflammatory disease mostly caused by oxidation of low density lipoproteins (LDLs) while High-density lipoproteins (HDLs) oppose atherosclerosis by inhibiting the oxidation of LDLs. Serum concentration of C- reactive protein (CRP) also increases as an acute phase protein in inflammatory conditions like atherosclerosis. We aimed to evaluate the relationship between serum levels of HDL and CRP in patients with atherosclerosis disease.
Material and Methods: CRP and HDL in 45 patients as a case and 45 healthy individuals as a control group were measured in the central laboratory of Yazd city. The CRP was measured by turbidometric quantitative method and HDL by colorimetric method.
Results: In case group, the CRP level was 7.62 ± 4.08 mg/l and the HDL level was 45.29 ± 9.41mg/dl, which are inversely correlated (P-value: 0.001, Pearson correlation: -0.700), while the correlation in control group was not significant (P-value: 0.88, Pearson correlation: 0.023).
Conclusion: Based on the resuls, there is a significant inverse correlation between CRP and HDL in atherosclerotic patients.
Keywords: Atherosclerosis Disease, CRP, HDL
Soltan Dallal, Mm, Rahbar, M, Douraghi, M, Rahimi Forooshani, A, Khan Babaei, Gt, Mobarhan, M, Ghasemi, F,
Volume 8, Issue 5 (1-2015)
Abstract
Abstract
Background and Objective: Cystic fibrosis (CF) is an autosomal recessive genetic disease and Pseudomonas aeruginosa is one of the most common bacteria colonized in CF patients. Growing resistance of this bacterium to antibiotics now a day is a challenge of controlling infection in CF patient. In this study colonization of CF patients with Pseudomonas aeruginosa and antibiotic susceptibility pattern of isolated strains were examined.
Material and Methods: From 100 CF patients, during a year, sputum and bronchial swabs were collected. After culturing the samples, some of them were reported as Pseudomonas aeruginosa using biochemical tests. Mucoid strains of Pseudomonas aeruginosa were identified the same as non-producing alginate strains while for catching single pure colony, repeated passage was used. For determining antibiotic resistance of Pseudomonas aeruginosa to some antimicrobial agents Kirby-Bauer method based on CLSI was used.
Results: Of 100 samples, 40 (40%) were positive for Pseudompnas aeruginosa. The prevalence of P. aeruginosa was 23.8, 36.84 and 80% at the age of 1-3, 4-12 and 13, respectively.
Conclusion: Statistically, there is a significant difference between age and contracting with Pseudomonas aeruginosa in that the higher the age the more colonization with Pseudomonas aeruginosa.
Key words: Pseudomonas Aeruginosa, Cystic Fibrosis, Drug Resistance
Roohi, Z, Moradi Bidhendi, S, Khaki, P,
Volume 9, Issue 1 (4-2015)
Abstract
Abstract
Background and Objective: Leptospirosis is a zoonosis infectious disease that is prevalent in tropical and subtropical regions and is caused by the pathogenic serovars of leptospires. Hence, we aimed at investigating the prevalence of antibodies against these bacteria in the blood samples of suspected leptospirosis.
Material and Methods: the human serum samples (N = 130) were obtained from patients clinically suspected leptospirosis. The Serum level of IgM antibodies were studied by ELISA kit (PrioCHECK) in Razi Vaccine and Serum Research Institute (Karaj), 2011-2012.
Results: Anti-leptospira IgM class was observed in 21(16%) samples. The relative distribution of the disease was reported in men (80.95%), women (19.04%), and farmers (30.95%) and in 20-40-year group (57.14%). Contact with contaminated water was the most common cause of infection (52.38%) and fever was the most common sign of Leptospirosis (72.2%).
Conclusion: Due to the occurrence of anti-leptospira antibodies in 16% of suspected cases, it is recommended that routine ELISA be done at least in major diagnostic centers.
Keywords: Leptospira, Leptospirosis, Human, ELISA
Seyyed Manuchehr Nourazarian , Mojtaba Ghaffarian , Homayun Dolatkhah ,
Volume 10, Issue 3 (5-2016)
Abstract
ABSTRACT
Background and Objective: The relationship between diabetes mellitus and increased risk of cardiovascular diseases has been demonstrated. The aim of this study was to determine the fatty acid profile of epicardial adipose tissue in diabetic and non-diabetic patients with cardiovascular disease.
Methods: In this study, 28 diabetic and 40 nondiabetic patients were evaluated. The epicardial adipose tissue and blood samples of patients were collected during surgery. Non‐esterified fatty acids and phospholipids were measured by the thin layer chromatography and gas chromatography.
Results: Saturated free fatty acids (12: 0) level was higher in diabetic patients compared to nondiabetic patients (P=0.038), while saturated free fatty acids (16: 0) was significantly lower in diabetics (P=0.011). Unsaturated fatty acid (20: 3n-9) was higher in nondiabetics compared to diabetics (P=0.015). There was a significant decrease in level of monounsaturated fatty acids in diabetic patients. The epicardial adipose tissue of diabetics showed a significant increase in free fatty acid (18: 0) and conjugated linoleic acid levels, while there was a significant decrease in the level of free fatty acid (18: 1n-11). Level of epicardial omega-3 free fatty acid (20: 5 n-3) and 22: 6 n -3 was significantly reduced in these patients.
Conclusion: Differences in the serum free fatty acid profile of the two groups may be due to differences in their diet, while changes in the fatty acid composition of epicardial adipose tissue in these two groups could be due to impaired metabolism of fatty acids such as uptake, movement and androgen synthesis as a result of diabetes. These changes increase the risk of developing atherosclerosis in diabetic patients.
Roya Rafiee , Fereshte Eftekhar , Seyed Ahmad Tabatabaei , Dariush Minaee-Tehrani ,
Volume 10, Issue 3 (5-2016)
Abstract
ABSTRACT
Background and Objectives: Pseudomonas aeruginosa is the most frequent opportunistic pathogen isolated from the sputum of patients with cystic fibrosis (CF). Resistance to β -lactam antibiotics may arise from over expression of the naturally occurring AmpC cephalosporinases or acquired extended-spectrum β-lactamases (ESBL). The aim of this study was to determine the antibiotic resistance profiles as well as the prevalence of ESBL and AmpC production in clinical isolates of P. aeruginosa from CF patients in Tehran.
Methods: Antibiotic resistance of 50 non-duplicate P. aeruginosa isolates was determined by the disc diffusion method. AmpC β-lactamase production was detected by the antagonism disc test and ESBL production was detected by the phenotypic confirmatory test. The presence of ESBL and AmpC genes was assessed by PCR, followed by sequencing the PCR products.
Results: The antibiotic resistance rates were as follows: 22% to ceftriaxone, 20% to cefotaxime, 10% to imipenem, 8% to carbenicillin and 6% to ticarcillin, 4% each to cefepime, tobramycin, amikacin and aztreonam and 2% to each piperacilin, meropenem and ceftazidime. AmpC production was observed in 47 isolates (94%) and ESBL production was observed in one isolate (2%). PCR results showed that all isolates carried the blaAmpC β-lactamase gene. One multidrug-resistant isolate carried both blaTEM and blaPER-1 genes.
Conclusion: The results showed that despite the low rate of antibiotic resistance in P. aeruginosa CF isolates,the presence of multiple β-lactamases even in one isolate is alarming and can complicate the already difficult treatment of chronic infections in the lungs of CF patients.
Haleh Foroutan , Marziyeh Khodabakhsh , Masoud Moharamzadeh ,
Volume 10, Issue 4 (7-2016)
Abstract
ABSTRACT
Background and Objective: Prostate specific antigen (PSA) is considered as one of the most reliable biomarkers of cancer and other known prostate diseases. In the present study, solid phase sandwich immunoradiometric assay was used to measure the amount of PSA. In this type of measurement, a pair of anti-PSA antibodies on the solid phase and labeled with Iodine-125, participate in forming a complex with two different epitopes of PSA.
Methods: Variables such as irradiation level, modification of polymer surfaces by alcohol washing, different concentrations and volumes of antibody, incubation temperature and drying conditions that influence the direct coating process were optimized. Finally, the stability, accuracy and precision of the laboratory kit were evaluated by comparison with a foreign kit.
Results: According to the obtained results, preliminary preparations such as irradiation, tube washing and specific temperature conditions are not required during the coating process. Drying by lyophilization method does not affect the quality of coating. Antibody concentration of 2.5 μg/ml and coating volume of 800 μl were determined as the optimum conditions for coating, which had good stability within a year. Alignment of results obtained from the domestic and foreign kits for accuracy of 30 samples from patients was confirmed by T-test (sig 2-tailed= 0.993 and 95% confidence interval). The short-term and long-term precision for three control ranges (low, medium, high) were less than 0.25 and 0.33 of allowable total error (TEa = 10%), respectively.
Conclusion: The produced domestic kit has acceptable precision according to the CLIA criteria.
Keywords: Biological testing, Radioimmunometric assay, monoclonal antibody, prostate specific antigen, prostate disease.
Huda Jomma Elkheer Jomma, Mohmmed Omer Abaker Gibreel,
Volume 11, Issue 2 (3-2017)
Abstract
ABSTRACT
Background and Objective: This descriptive study aimed to evaluate the presence and frequency distribution of mosquito species in the city of Port Sudan, Sudan.
Methods: After collection of 500 adult mosquitoes from different sectors of the city Port Sudan, they were classified using the morphological keys for identification of mosquitoes.
Results: Overall, 230
Culex quinquefasciatus (46%) were detected in the eastern, central and southern sectors of the city.
Culex sitiens (14.2%) were detected only in the central sector.
Aedes aegypti (32.2%) were detected in all three sectors.
Anopheles funestus (6.6%) were detected in the central and southern sectors.
Conclusion: Cx. quinquefasciatus and
Ae. aegypi are prevalent in all sectors of the city, while
Cx. sitiens is prevalent only in the central sector.
An. funestus is abundant in the central and southern sectors.
Keywords: Culicidae,
Morphological and Microscopic Findings, Eastern Sudan.
Mehdi Tavalla , Marjan Sabzevari,
Volume 11, Issue 3 (5-2017)
Abstract
ABSTRACT
Background and Objective: Toxoplasmosis is a common parasitic infection worldwide. The infection can be caused via consumption of contaminated meat and mother-to-child (congenital) transmission, causing changes in central nervous system tissue, eye irritation and sometimes death. The human form of the disease is often asymptomatic and may be accompanied with general discomfort and swelling of the lymph nodes when associated with chorioretinitis. Acute infection in immunocompromised individuals could lead to mortality. The purpose of this study was to determine the prevalence of anti-Toxoplasma gondii antibodies in serum of patients with multiple sclerosis (MS) referred to the MS Center in city of Ahvaz, southeastern Iran.
Methods: Blood samples were taken from 100 patients with MS and 100 healthy control participants. After separating the serum, presence of anti-Toxoplasma antibodies (IgG, IgM) was evaluated by enzyme-linked immunosorbent assay.
Results: Frequency of anti-Toxoplasma IgG was 38% and 21% in the patients and controls, respectively. Toxoplasma IgM antibodies were not detected in any of the study groups. Pearson correlation coefficient showed a significant association between Toxoplasma antibodies and MS.
Conclusion: Due to high prevalence of toxoplasmosis in MS patients, it is recommended to measure serum titers of the patients regularly, and placed them under antiparasitic therapy when necessary.
Keywords: Toxoplasma, Multiple sclerosis, MS, Ahvaz.
